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1x b-27 supplement without insulin  (Thermo Fisher)


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    Thermo Fisher 1x b-27 supplement without insulin
    1x B 27 Supplement Without Insulin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/b-27+without+insulin+supplement/1x+b+27+supplement+without+insulin/bio_rxiv__2025__04__18__649376-57-6-11
    Average 90 stars, based on 1 article reviews
    1x b-27 supplement without insulin - by Bioz Stars, 2026-09
    90/100 stars

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    Cell Culture:

    Article Title: Scalable Generation of Nanovesicles from Human-Induced Pluripotent Stem Cells for Cardiac Repair.
    Article Snippet: .. At day 2, medium was changed to RPMI 1640 basal medium containing B-27 without insulin supplement and 5 μM IWP-2 (Sigma-Aldrich, Burlington, MA, USA) for 72 h. From day 5, cells were cultured in RPMI 1640 basal medium containing B-27 supplement (Thermo Fisher Scientific) and 200 μg/mL L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma-Aldrich) (cardiomyocyte medium, CMm), with CMm changed every 2–3 days. .. At day 12, differentiated cardiomyocytes were dissociated into single cells and split 1:4 onto human embryonic stem cell qualified-Matrigel coated plates in DMEM/F-12 GlutaMAX medium supplemented with 20% FBS (Sigma-Aldrich), 0.1 mM 2-mercaptoethanol, 0.1 mM nonessential amino acids, 50 U/mL penicillin/streptomycin and 10 μM Y-27632.

    Article Title: MAGIK: A rapid and efficient method to create lineage-specific reporters in human pluripotent stem cells
    Article Snippet: .. From Day 1 to Day 4, cells were cultured in RPMI containing 2% v/v B-27 without insulin supplement (Thermo Fisher) with daily media changes. ..

    Article Title: A novel small molecule inhibitor of human Drp1.
    Article Snippet: .. At day 2, medium was changed to RPMI 1640 basal medium containing B-27 without insulin supplement and 5 μM IWP-2 (Sigma-Aldrich) for 72 h. From day 5, cells were cultured in cardiomyocyte medium containing RPMI 1640 basal medium, B-27 supplement (Thermo Fisher Scientific) and 200 μg/mL L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma-Aldrich). .. At day 12, differentiated cardiomyocytes were dissociated into single cells and split 1:2 onto Matrigel coated plates in DMEM/F-12 GlutaMAX medium supplemented with 20% FBS (Sigma-Aldrich), 0.1 mM 2-mercaptoethanol, 0.1 mM nonessential amino acids, 50 U/ mL penicillin/streptomycin and 10 μM Y−27632.



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